PuSH - Publikationsserver des Helmholtz Zentrums München

Two antisense promoters in the immunoglobulin μ-switch region drive expression of c-myc in the Burkitt's lymphoma cell line BL67.

Oncogene 7, 1267-1271 (1992)
Verlagsversion DOI
Free by publisher
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
In the Burkitt's lymphoma (BL) cell line BL67 the first exon of the c-myc gene is fused to the μ-switch region of the immunoglobulin heavy-chain gene (IgH). BL67 cells express IgH/c-myc hybrid RNAs which are initiated in the immunoglobulin locus, transcribed across the chromosomal breakpoint into the first exon of c-myc and spliced using the physiological splice donor and acceptor sites of the c-myc gene. We have isolated cDNAs of these hybrid RNAs and characterized the start points in the Ig heavy-chain gene. Two promoters were identified in the μ-switch region of BL67 cells which give rise to antisense transcription of the μ-gene. These promoters are also active in other BL cell lines, in B cells without Ig translocation and in a T-cell line. Both promoters co-localize with DNAase I-hypersensitive sites, HNF and HSW, in the μ-switch region. The structures of IgH/c-myc hybrid RNAs and of the corresponding promoters are described.
Weitere Metriken?
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
ISSN (print) / ISBN 0950-9232
e-ISSN 0950-9232
Zeitschrift Oncogene
Quellenangaben Band: 7, Heft: 7, Seiten: 1267-1271 Artikelnummer: , Supplement: ,
Verlag Nature Publishing Group
Begutachtungsstatus Peer reviewed