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Species and tissue specificity of mammalian GTP cyclohydrolase I messenger RNA.

Biochim. Biophys. Acta 1171, 133-140 (1992)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
Northern blot analysis of rat RNA from cell lines and isolated organs with a specific rat cDNA probe detected two GTP cyclohydrolase I mRNA species of approx. 1.4 and 3.6 kb. The ratio between these two species varies between 0.6 and 2.4 in different rat organs. Using primers derived from highly conserved regions in the rat and Escherichia coli cDNA sequences a human GTP cyclohydrolase I probe was obtained by means of reverse transcription and PCR (polymerase chain reaction). The human PCR product consisting of 555 bp was cloned and sequenced. It shows a 92% identity with the published sequence of the rat gene. The analysis of various human cell lines with this specific probe shows only one species of GTP cyclohydrolase I mRNA with an approximate size of 3.6 kb.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Gtp Cyclohydrolase I ; Northern Blot ; Polymerase Chain Reaction ; Tetrahydrobiopterin
ISSN (print) / ISBN 0006-3002
Quellenangaben Volume: 1171, Issue: 2, Pages: 133-140 Article Number: , Supplement: ,
Publisher Elsevier