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Winkelheide, U.* ; Engelhard, K.* ; Kaeppel, B.* ; Winkler, J.* ; Hutzler, P. ; Werner, C.* ; Kochs, E.*

Cerebral Ischemia and Neurogenesis: A Two-time Comparison.

Neurocrit. Care 9, 387-393 (2008)
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
This study compares the effect of mild and severe cerebral ischemia on neuronal damage and neurogenesis. METHODS: Sixteen Sprague-Dawley rats, anesthetized with 0.8 vol% halothane in O(2)/air, were subjected to forebrain ischemia by bilateral common carotid artery occlusion plus hemorrhagic hypotension (mean arterial blood pressure = 40 mmHg) for 8 (mild) or 13 (severe) min. Four non-ischemic animals were investigated as naïve controls. Bromodeoxyuridine (50 mg/kg), a marker of new cells, was administrated for seven consecutive postischemic days. After 28 days, animals were perfused with 4% paraformaldehyde and the brains were sliced. Histopathological damage of the hippocampus and the volume of the dentate gyrus were assessed by HE-staining. With immunohistochemistry BrdU-positve cells were detected in the dentate gyrus. The amount of new generated neurons was identified by double-immunofluorescence-staining of BrdU and neuronal marker (NeuN). RESULTS: In the CA-1 region of the hippocampus, mild ischemia induced damage up to 10% (HE-index 0.8 +/- 1.2) and severe ischemia up to 50% (HE-index 2.1 +/- 1.4). There was no histopathological damage in naïve control animals. The amount of new neurons was increased by 250% after mild insult and by 160% after severe insult compared to the naïve control animals. CONCLUSIONS: These data indicate that histopathological damage depends on the severity of the ischemic insult and that forebrain ischemia activates generation of new neurons. A mild ischemic challenge appears to be a more potent neurogenic stimulus than severe ischemia. The new neurons survive at least 28 days. This may relate to delayed histopathological and functional recovery after cerebral ischemia.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Neurogenesis; Dentate gyrus; Insult severity; Cerebral ischemia
ISSN (print) / ISBN 1541-6933
e-ISSN 1556-0961
Zeitschrift Neurocritical Care
Quellenangaben Band: 9, Heft: 3, Seiten: 387-393 Artikelnummer: , Supplement: ,
Verlag Springer
Begutachtungsstatus Peer reviewed