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Genome-wide analysis of caesium and strontium accumulation in Saccharomyces cerevisiae.

Yeast 27, 817-835 (2010)
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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
(137)Cs and (90)Sr contribute to significant and long-lasting contamination of the environment with radionuclides. Due to their relatively high biological availability, they are transferred rapidly into biotic systems and may enter the food chain. In this study, we analysed 4862 haploid yeast knockout strains of Saccharomyces cerevisiae to identify genes involved in caesium (Cs(+)) and/or strontium (Sr(2+)) accumulation. According to this analysis, 212 mutant strains were associated with reproducible altered Cs(+) and/or Sr(2+) accumulation. These mutants were deficient for a wide range of cellular processes. Among those, the vacuolar function and biogenesis turned out to be crucial for both Cs(+) and Sr(2+) accumulation. Disruption of the vacuole diminished Cs(+) accumulation, whereas Sr(2+) enrichment was enhanced. Further analysis with a subset of the identified candidates were undertaken comparing the accumulation of Cs(+) and Sr(2+) with their essential counterparts potassium (K(+)) and calcium (Ca(2+)). Sr(2+) and Ca(2+) accumulation was highly correlated in yeast excluding the possibility of a differential regulation or uptake mechanisms. In direct contrast, the respective results suggest that Cs(+) uptake is at least partially dependent on mechanisms distinct from K(+) uptake. Single candidates (e.g. KHA1) are presented which might be specifically responsible for Cs(+) homeostasis.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Caesium; Strontium; Ion homeostasis; Comparison of essential and nonessential ions
ISSN (print) / ISBN 0749-503X
e-ISSN 1097-0061
Zeitschrift Yeast
Quellenangaben Band: 27, Heft: 10, Seiten: 817-835 Artikelnummer: , Supplement: ,
Verlag Wiley
Institut(e) Institute of Radiation Protection (ISS)
Institute of Ecological Chemistry (IOEC)