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Isolation of glutathione-deficient mutants of the yeast Saccharomyces cerevisiae.
Mutat. Res. 173, 117-120 (1986)
Glutathione-deficient (gsh-) mutants of the yeast Saccharomyces cerevisiae were isolated after UV treatment using MNNG as selective agent. For genetic and biochemical characterization 5 mutant strains were chosen which exhibited considerably decreased residual GSH contents varying from 2 to 6% of the wild-type levels. All 5 isolates showed a 2:2 segregation of the gsh-:GSH+ phenotypes alluding to a monogenic recessive mutation. Complementation analysis indicates that all gsh- mutants belong to one complementation group.
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Publication type Article: Journal article
Document type Scientific Article
Institute(s) Abteilung für Strahlenbiologie und Biophysik