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Sim, N.* ; Pal, R.* ; Parker, D.J.* ; Engelmann, J.* ; Mishra, A. ; Gottschalk, S.

Magnetic resonance and optical imaging probes for NMDA receptors on the cell surface of neurons: Synthesis and evaluation in cellulo.

Org. Biomol. Chem. 12, 9389-9404 (2014)
DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
A second generation of N-methyl-d-aspartate (NMDA) receptor-targeted MRI contrast agents has been synthesised and evaluated in cellulo, based on established bicyclic NMDA receptor antagonists. Their use as responsive MR imaging probes has been evaluated in suspensions of NSC-34 cells, and one agent exhibited significant enhancements in measured longitudinal and transverse water proton relaxation rates (19 and 38% respectively; 3 T, 298 K). A biotin derivative of the lead compound was prepared and the specificity and reversibility of binding to the NMDA cell surface receptors demonstrated using confocal laser scanning microscopy. Competitive and reversible binding of glutamate to the receptors was also visualised, suggesting that the receptor-targeted approach may allow MRI to be used to monitor neuronal events associated with modulation of local glutamate concentrations.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
ISSN (print) / ISBN 1477-0520
e-ISSN 1477-0539
Quellenangaben Band: 12, Heft: 46, Seiten: 9389-9404 Artikelnummer: , Supplement: ,
Verlag Royal Society of Chemistry (RSC)
Begutachtungsstatus Peer reviewed