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Chevelkov, V.* ; Xiang, S.* ; Giller, K.* ; Becker, S.T.* ; Lange, A.D.* ; Reif, B.

Perspectives for sensitivity enhancement in proton-detected solid-state NMR of highly deuterated proteins by preserving water magnetization.

J. Biomol. NMR 61, 151-160 (2015)
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In this work, we show how the water flip-back approach that is widely employed in solution-state NMR can be adapted to proton-detected MAS solid-state NMR of highly deuterated proteins. The scheme allows to enhance the sensitivity of the experiment by decreasing the recovery time of the proton longitudinal magnetization. The method relies on polarization transfer from non-saturated water to the protein during the inter-scan delay.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Magic Angle Spinning (mas) Solid-state Nmr ; Perdeuteration ; Protein Water Interaction ; Sensitivity Enchancement; Angle-spinning Nmr; Resonance Assignment; Cross-polarization; Hsqc Spectra; Flip-back; Spectroscopy; Resolution; Relaxation; Excitation; Dynamics
ISSN (print) / ISBN 0925-2738
e-ISSN 1573-5001
Quellenangaben Band: 61, Heft: 2, Seiten: 151-160 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort Dordrecht
Begutachtungsstatus Peer reviewed