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High-contrast imaging of reversibly switchable fluorescent proteins via temporally unmixed multispectral optoacoustic tomography.

Opt. Lett. 40, 367-370 (2015)
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Photocontrol of reversibly switchable fluorescent proteins (RSFPs) was used to program optoacoustic signal time courses that were temporally unmixed to increase the proteins' contrast-to-noise-ratios (CNRs) in optoacoustic imaging. In this way, two variants of the RSFP Dronpa with very similar optoacoustic spectra could be readily discriminated in the presence of highly absorbing blood. Addition of temporal unmixing to multispectral optoacoustic tomography (tuMSOT) in conjunction with synthetic or genetically encoded photochromic contrast agents and customized photoswitching schedules can increase the performance of multiplexed and high-contrast molecular optoacoustic imaging.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Vertex Component Analysis; Stimulated-emission; Microscopy; Gfp; Dronpa; Cells; Limit
ISSN (print) / ISBN 0146-9592
e-ISSN 1539-4794
Journal Optics Letters
Quellenangaben Volume: 40, Issue: 3, Pages: 367-370 Article Number: , Supplement: ,
Publisher Optical Society of America (OSA)
Publishing Place Washington
Reviewing status Peer reviewed