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Ivanova, A.* ; Kalaidzidis, Y.* ; Dirkx, R.* ; Sarov, M.* ; Gerlach, M.* ; Schroth-Diez, B.* ; Müller, A.* ; Liu, Y.* ; Andree, C.* ; Mulligan, B.* ; Münster, C.* ; Kurth, T.* ; Bickle, M.* ; Speier, S.* ; Anastassiadis, K.* ; Solimena, M.*

Age-dependent labeling and imaging of insulin secretory granules.

Diabetes 62, 3687-3696 (2013)
DOI Order publishers version
Open Access Green as soon as Postprint is submitted to ZB.
Insulin is stored within the secretory granules of pancreatic β-cells, and impairment of its release is the hallmark of type 2 diabetes. Preferential exocytosis of newly synthesized insulin suggests that granule aging is a key factor influencing insulin secretion. Here, we illustrate a technology that enables the study of granule aging in insulinoma cells and β-cells of knock-in mice through the conditional and unequivocal labeling of insulin fused to the SNAP tag. This approach, which overcomes the limits encountered with previous strategies based on radiolabeling or fluorescence timer proteins, allowed us to formally demonstrate the preferential release of newly synthesized insulin and reveal that the motility of cortical granules significantly changes over time. Exploitation of this approach may enable the identification of molecular signatures associated with granule aging and unravel possible alterations of granule turnover in diabetic β-cells. Furthermore, the method is of general interest for the study of membrane traffic and aging.
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Publication type Article: Journal article
Document type Scientific Article
ISSN (print) / ISBN 0012-1797
e-ISSN 1939-327X
Journal Diabetes
Quellenangaben Volume: 62, Issue: 11, Pages: 3687-3696 Article Number: , Supplement: ,
Publisher American Diabetes Association
Publishing Place Alexandria, VA.
Reviewing status Peer reviewed
Institute(s) Institute for Pancreatic Beta Cell Research (IPI)