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Petzold, C.* ; Steinbronn, N.* ; Gereke, M.* ; Strasser, R.H.* ; Sparwasser, T.* ; Bruder, D.* ; Geffers, R.* ; Schallenberg, S.* ; Kretschmer, K.*

Fluorochrome-based definition of naturally occurring Foxp3+ regulatory T cells of intra- and extrathymic origin.

Eur. J. Immunol. 44, 3632-3645 (2014)
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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Under physiological conditions, studies on the biology of naturally induced Foxp3(+) Treg cells of intra- and extrathymic origin have been hampered by the lack of unambiguous markers to discriminate the mature progeny of such developmental Treg-cell sublineages. Here, we report on experiments in double-transgenic mice, in which red fluorescent protein (RFP) is expressed in all Foxp3(+) Treg cells, whereas Foxp3-dependent GFP expression is exclusively confined to intrathymically induced Foxp3(+) Treg cells. This novel molecular genetic tool enabled us to faithfully track and characterize naturally induced Treg cells of intrathymic (RFP(+) GFP(+) ) and extrathymic (RFP(+) GFP(-) ) origin in otherwise unmanipulated mice. These experiments directly demonstrate that extrathymically induced Treg cells substantially contribute to the overall pool of mature Foxp3(+) Treg cells residing in peripheral lymphoid tissues of steady-state mice. Furthermore, we provide evidence that intra- and extrathymically induced Foxp3(+) Treg cells represent distinct phenotypic and functional sublineages.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Fluorochrome Reporter ; Foxp3 ; Regulatory T Cells ; T-cell Development
ISSN (print) / ISBN 0014-2980
e-ISSN 1521-4141
Quellenangaben Band: 44, Heft: 12, Seiten: 3632-3645 Artikelnummer: , Supplement: ,
Verlag Wiley
Verlagsort Hoboken
Begutachtungsstatus Peer reviewed
Institut(e) Institute for Pancreatic Beta Cell Research (IPI)