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Labazi, M.* ; McNeil, A.K.* ; Kurtz, T.* ; Lee, T.C.* ; Pegg, R.B.* ; Friedmann Angeli, J.P.F. ; Conrad, M. ; McNeil, P.L.*

The antioxidant requirement for plasma membrane repair in skeletal muscle.

Free Radical Biol. Med. 84, 246-253 (2015)
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Vitamin E (VE) deficiency results in pronounced muscle weakness and atrophy but the cell biological mechanism of pathology is unknown. We previously showed that VE supplementation promotes membrane repair in cultured cells and that oxidants potently inhibit repair. Here we provide three independent lines of evidence that VE is required for skeletal muscle myocyte plasma membrane repair in vivo. We also show that when another lipid-directed antioxidant, glutathione peroxidase 4 (Gpx4), is genetically deleted in mouse embryonic fibroblasts, repair fails catastrophically, unless cells are supplemented with VE. We conclude that lipid-directed antioxidant activity provided by VE, and possibly also Gpx4, is an essential component of the membrane repair mechanism in skeletal muscle. This work explains why VE is essential to muscle health and identifies VE as a requisite component of the plasma membrane repair mechanism in vivo.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Antioxidants ; Free Radicals ; Membrane Repair ; Skeletal Muscle ; Vitamin E; Hydroperoxide Glutathione-peroxidase; Frog Neuromuscular-junction; Vitamin-e Supplementation; Muscular-dystrophy; Oxidative Stress; E-deficiency; Exercise; Damage; Disruptions; Myopathy
ISSN (print) / ISBN 0891-5849
e-ISSN 1873-4596
Quellenangaben Volume: 84, Issue: , Pages: 246-253 Article Number: , Supplement: ,
Publisher Elsevier
Publishing Place New York, NY
Reviewing status Peer reviewed