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Bruns, H.* ; Büttner, M.* ; Fabri, M.* ; Mougiakakos, D.* ; Bittenbring, J.T.* ; Hoffmann, M.H.* ; Beier, F.* ; Pasemann, S.* ; Jitschin, R.* ; Hofmann, A.D.* ; Neumann, F.J.* ; Daniel, C.* ; Maurberger, A.* ; Kempkes, B. ; Amann, K.* ; Mackensen, A.* ; Gerbitz, A.*

Vitamin D-dependent induction of cathelicidin in human macrophages results in cytotoxicity against high-grade B cell lymphoma.

Sci. Transl. Med. 7:282ra47 (2015)
Open Access Green as soon as Postprint is submitted to ZB.
Infiltration by macrophages represents a characteristic morphological hallmark in high-grade lymphatic malignancies such as Burkitt's lymphoma (BL). Although macrophages can, in principle, target neoplastic cells and mediate antibody-dependent cellular cytotoxicity (ADCC), tumor-associated macrophages (TAMs) regularly fail to exert direct cytotoxic functions. The underlying mechanisms responsible for this observation remain unclear. We demonstrate that inflammatory M1 macrophages kill proliferating high-grade B cell lymphoma cells by releasing the antimicrobial peptide cathelicidin in a vitamin D-dependent fashion. We show that cathelicidin directly induces cell death by targeting mitochondria of BL cells. In contrast, anti-inflammatory M2 macrophages and M2-like TAMs in BL exhibit an altered vitamin D metabolism, resulting in a reduced production of cathelicidin and consequently in inability to lyse BL cells. However, treatment of M2 macrophages with the bioactive form of vitamin D, 1,25D3, or a vitamin D receptor agonist effectively induces cathelicidin production and triggers tumoricidal activity against BL cells. Furthermore, rituximab-mediated cytotoxicity of vitamin D-treated M2 macrophages is cathelicidin-dependent. Finally, vitamin D treatment of 25-hydroxyvitamin D (25D)-deficient volunteers in vivo or primary TAMs in vitro improves rituximab-mediated ADCC against B cell lymphoma cells. These data indicate that activation of the vitamin D signaling pathway activates antitumor activity of TAMs and improves the efficacy of ADCC.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Antimicrobial Peptide Ll-37; Non-hodgkins-lymphoma; Tumor-associated Macrophages; Bacillus-calmette-guerin; Human-monocytes; Monoclonal-antibody; Cancer Progression; Innate Immunity; D-receptor; Rituximab
ISSN (print) / ISBN 1946-6234
e-ISSN 1946-6242
Quellenangaben Volume: 7, Issue: 282, Pages: , Article Number: 282ra47 Supplement: ,
Publisher American Association for the Advancement of Science (AAAS)
Publishing Place Washington
Reviewing status Peer reviewed