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Stengel, A. ; Bach, C. ; Vorberg, I.* ; Frank, O.* ; Gilch, S.* ; Lutzny, G.* ; Seifarth, W.* ; Erfle, V. ; Maas, E.* ; Schätzl, H.* ; Leib-Mösch, C. ; Greenwood, A.D.

Prion infection influences murine endogenous retrovirus expression in neuronal cells.

Biochem. Biophys. Res. Commun. 343, 825-831 (2006)
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Prions as causative agents of transmissible spongiform encephalopathies have been well investigated in experimental and modelling work. However, little is known about the molecular pathogenesis of prion-induced encephalopathies, the role of co-factors, and the interaction of prions with cellular components. We investigated the influence of prion infection on expression of murine endogenous retroviruses (ERVs), which compose approximately 10% of the mouse genome. Hypothalamic neuronal cells (GT1) and neuroblastoma cells (N2a) were examined. Both cell lines can be persistently infected with mouse adapted prion strains, i.e., RML. Using a mammalian retrovirus-specific DNA microarray and quantitative PCR methods, we compared the expression profiles of ERVs in prion-infected, uninfected, and anti-prion compound-treated murine neuronal cell lines, including clonal cell populations. The results suggest that prion infection influences ERV expression in neuronal cell lines, that this influence is cell line-specific, ERV-specific, and responsive to anti-prion compound treatment.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter endogenous retrovirus; prions; neuron; DNA microarray; GT1
ISSN (print) / ISBN 0006-291X
e-ISSN 1090-2104
Quellenangaben Band: 343, Heft: , Seiten: 825-831 Artikelnummer: , Supplement: ,
Verlag Elsevier
Begutachtungsstatus Peer reviewed