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Wimmer, M. ; Alessandrini, F. ; Gilles, S.* ; Frank, U. ; Oeder, S. ; Hauser, M.* ; Ring, J.* ; Ferreira, F.* ; Ernst, D. ; Winkler, J.B. ; Schmitt-Kopplin, P. ; Ohnmacht, C.* ; Behrendt, H. ; Schmidt-Weber, C.B. ; Traidl-Hoffmann, C.* ; Gutermuth, J.

Pollen-derived adenosine is a necessary co-factor for ragweed allergy.

Allergy 70, 944-954 (2015)
Verlagsversion Postprint DOI
Open Access Green
BACKGROUND: Ragweed (Ambrosia artemisiifolia) is a strong elicitor of allergic airway inflammation with worldwide increasing prevalence. Various components of ragweed pollen are thought to play a role in the development of allergic responses. Aim of the study was to identify critical factors for allergenicity of ragweed pollen in a physiologic model of allergic airway inflammation METHODS: Aqueous ragweed pollen extract, the low molecular weight fraction or the major allergen Amb a 1 were instilled intranasally on 1 - 11 consecutive days and allergic airway inflammation was evaluated by bronchoalveolar lavage, lung histology, serology, gene-expression in lung tissue and measurement of lung function. Pollen-derived adenosine was removed from the extract enzymatically in order to analyze its role in ragweed-induced allergy. Migration of human neutrophils and eosinophils towards supernatants of ragweed-stimulated bronchial epithelial cells was analyzed RESULTS: Instillation of ragweed pollen extract, but not of the major allergen or the low molecular weight fraction, induced specific IgG1 , pulmonary infiltration with inflammatory cells, a Th2-associated cytokine signature in pulmonary tissue and impaired lung function. Adenosine aggravated ragweed-induced allergic lung inflammation. In vitro, human neutrophils and eosinophils migrated towards supernatants of bronchial epithelial cells stimulated with ragweed extract only if adenosine was present CONCLUSIONS: Pollen-derived adenosine is a critical factor in ragweed-pollen induced allergic airway inflammation. Future studies aim at therapeutic strategies to control these allergen-independent pathways.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Adenosine ; Adjuvant ; Allergic Inflammation ; Ragweed ; Sensitization; Airway Inflammation; Lung Inflammation; Immune-responses; Mouse Model; Pulmonary Inflammation; Lipid Mediators; V 1; Receptor; Asthma; Mice
ISSN (print) / ISBN 0105-4538
e-ISSN 1398-9995
Zeitschrift Allergy
Quellenangaben Band: 70, Heft: 8, Seiten: 944-954 Artikelnummer: , Supplement: ,
Verlag Wiley
Verlagsort Hoboken
Begutachtungsstatus Peer reviewed