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MPP1 as a factor regulating phase separation in giant plasma membrane-derived vesicles.

Biophys. J. 108, 2201-2211 (2015)
Publishers Version DOI PMC
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The existence of membrane-rafts helps to conceptually understand the spatiotemporal organization of membrane-associated events (signaling, fusion, fission, etc.). However, as rafts themselves are nanoscopic, dynamic, and transient assemblies, they cannot be directly observed in a metabolizing cell by traditional microscopy. The observation of phase separation in giant plasma membrane-derived vesicles from live cells is a powerful tool for studying lateral heterogeneity in eukaryotic cell membranes, specifically in the context of membrane rafts. Microscopic phase separation is detectable by fluorescent labeling, followed by cooling of the membranes below their miscibility phase transition temperature. It remains unclear, however, if this lipid-driven process is tuneable in any way by interactions with proteins. Here, we demonstrate that MPP1, a member of the MAGUK family, can modulate membrane properties such as the fluidity and phase separation capability of giant plasma membrane-derived vesicles. Our data suggest that physicochemical domain properties of the membrane can be modulated, without major changes in lipid composition, through proteins such as MPP1.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Tight Junction Protein; Maguk Family; Physiological Temperature; Promotes Synaptogenesis; Immunological Synapse; Cell Polarity; Lipid Rafts; T-cells; Palmitoylation; Domains
Reviewing status
Institute(s) Institute for Pancreatic Beta Cell Research (IPI)