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Amort, M.* ; Nachbauer, B.* ; Tuzlak, S.* ; Kieser, A. ; Schepers, A. ; Villunger, A.* ; Polacek, N.*

Expression of the vault RNA protects cells from undergoing apoptosis.

Nat. Commun. 6:7030 (2015)
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Non-protein-coding RNAs are a functionally versatile class of transcripts exerting their biological roles on the RNA level. Recently, we demonstrated that the vault complex-associated RNAs (vtRNAs) are significantly upregulated in Epstein-Barr virus (EBV)-infected human B cells. Very little is known about the function(s) of the vtRNAs or the vault complex. Here, we individually express latent EBV-encoded proteins in B cells and identify the latent membrane protein 1 (LMP1) as trigger for vtRNA upregulation. Ectopic expression of vtRNA1-1, but not of the other vtRNA paralogues, results in an improved viral establishment and reduced apoptosis, a function located in the central domain of vtRNA1-1. Knockdown of the major vault protein has no effect on these phenotypes revealing that vtRNA1-1 and not the vault complex contributes to general cell death resistance. This study describes a NF-κB-mediated role of the non-coding vtRNA1-1 in inhibiting both the extrinsic and intrinsic apoptotic pathways.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Epstein-barr-virus; Caspase Recruitment Domain; Noncoding Rna; Multidrug-resistance; Burkitts-lymphoma; Malignant-cells; Cancer-cells; Death; Repressor; Dna
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Quellenangaben Volume: 6, Issue: , Pages: , Article Number: 7030 Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Reviewing status Peer reviewed