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Quantification of regenerative potential in primary human mammary epithelial cells.

Development 142, 3239-3251 (2015)
Publishers Version DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
as soon as is submitted to ZB.
We present an organoid regeneration assay in which freshly isolated human mammary epithelial cells are cultured in adherent or floating collagen gels, corresponding to a rigid or compliant matrix environment. In both conditions, luminal progenitors form spheres, whereas basal cells generate branched ductal structures. In compliant but not rigid collagen gels, branching ducts form alveoli at their tips, express basal and luminal markers at correct positions, and display contractility, which is required for alveologenesis. Thereby, branched structures generated in compliant collagen gels resemble terminal ductal-lobular units (TDLUs), the functional units of the mammary gland. Using the membrane metallo-endopeptidase CD10 as a surface marker enriches for TDLU formation and reveals the presence of stromal cells within the CD49f(hi)/EpCAM(-) population. In summary, we describe a defined in vitro assay system to quantify cells with regenerative potential and systematically investigate their interaction with the physical environment at distinct steps of morphogenesis.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Basal ; Branching Morphogenesis ; Cd10 ; Collagen Gel ; Luminal ; Mammary Stem Cell ; Organoid ; Primary Human Mammary Epithelial Cells ; Progenitor Cells ; Regenerative Potential ; Terminal Ductal-lobular Units
Reviewing status