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In vivo retrograde labeling of neurons in the zebrafish embryo or larva with rhodamine dextran.

Cold Spring Harb. Protoc. 7, pdb.prot4832 (2007)
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Application of a fluorescent dye to a target area of axons or dendrites allows the identification of individual neurons projecting to the area and reconstruction of the tract of the neurite by retrograde labeling. For the red fluorescent dye tetramethylrhodamine dextran, the labeling occurs by active retrograde transport inside the cell. This protocol describes a method for applying tetramethylrhodamine dextran to living zebrafish embryos and larvae (2-7 dpf [days post-fertilization]) via microinjection. It may be adapted for use with green or yellow fluorescent dextran conjugates.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
ISSN (print) / ISBN 1940-3402
e-ISSN 1559-6095
Quellenangaben Band: 7, Heft: , Seiten: pdb.prot4832 Artikelnummer: , Supplement: ,
Verlag Cold Spring Harbor Laboratory Press
Begutachtungsstatus Peer reviewed