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Muschaweckh, A. ; Gasteiger, G. ; Kastenmüller, W. ; König, P.A* ; Fischer, A.* ; Baier, R.* ; Drexler, I.

Intradermal tattoo vaccination enhances systemic and local immunogenicity of MVA vector vaccines.

Vortrag: 8th Workshop on Imunology of Viral Infections, GfV, 7-9 October 2009, Deidesheim, Germany. (2009)
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The skin, as the primary interface between the internal organs and the environment represents a first line of defence against invading pathogens and toxins. Apart from being a passive barrier, the skin also provides protection via a network of specialized innate and adaptive immune cells, blood vessels and lymphatics. For this reason, the skin constitutes a promising target tissue for vaccination. This has been demonstrated by the successful eradication of smallpox through skin-scarification with vaccinia viruses (VACV). Here, short-interval intradermal skin tattooing using recombinant MVA (modified vaccinia virus Ankara) expressing the model antigen ovalbumin (OVA) was tested for its capacity to induce cytotoxic T-cell (CTL) immunity in C57BL/6 mice. We found that despite a lower antigen expression at the site of infection, skin tattooing elicited a larger number of virus-specific effector T-cells than a single intradermal (i.d.) needle injection. Following adoptive transfer, the viral tattoo-induced strong proliferation of transferred OVA-specific T-cell receptor (TCR)-transgenic cells (OT-I cells) in the local skin-draining LN, expression of skin-homing surface-markers on responding T-cells as well as their recruitment to the site of vaccination. Interestingly, the vaccination induced skin-resident memory T-cells that are phenotypically different from their systemic and lymphoid counterparts. Moreover, mice immunized by tattooing were protected against a lethal challenge of replication-competent vaccinia virus (VACV) similarly to mice vaccinated via the conventional intramuscular route of virus application. The experiments show that intradermal delivery of MVA to the skin via tattoo might be a promising vaccination strategy to enhance the immunogenicity of recombinant MVA vaccines.
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Publikationstyp Sonstiges: Vortrag
Konferenztitel 8th Workshop on Imunology of Viral Infections, GfV
Konferzenzdatum 7-9 October 2009
Konferenzort Deidesheim, Germany