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A nuclear isoform of the focal adhesion LIM-domain protein Trip6 integrates activating and repressing signals at AP-1- and NK-kB-regulated promoters.
Genes Dev. 18, 2518-2528 (2004)
Glucocorticoid receptor (GR)-mediated transrepression of the transcription factors AP-1 and NF-kappaB, responsible for most of the anti-inflammatory effects of glucocorticoids, is initiated by the tethering of GR to the promoters of target genes. We report that this tethering is mediated by a nuclear isoform of the focal adhesion LIM domain protein Trip6. Trip6 functions as a coactivator for both AP-1 and NF-kappaB. As shown by chromatin immunoprecipitation, Trip6 is recruited to the promoters of target genes together with AP-1 or NF-kappaB. In the presence of glucocorticoids, GR joins the Trip6 complex. Reducing the level of Trip6 by RNA interference or abolishing its interaction with GR by dominant-negative mutation eliminates transrepression. We propose that GR tethering to the target promoter through Trip6 forms the basis of transrepression, and that Trip6 exerts its nuclear functions by acting as a molecular platform, enabling target promoters to integrate activating or repressing signals.
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Publication type Article: Journal article
Document type Scientific Article
Keywords glucocorticoids; LIM domains; transactivation; repression; chromatin immunoprecipitation
ISSN (print) / ISBN 0890-9369
Journal Genes and Development
Quellenangaben Volume: 18, Pages: 2518-2528
Publisher Cold Spring Harbor Laboratory Press
Reviewing status Peer reviewed