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Molecular cloning of ozone-inducible protein from Pinus sylvestris L. with high sequence similarity to vertebrate 3-hydroxy-3-methylglutaryl-CoA-synthase.
Biochim. Biophys. Acta 1350, 247-252 (1997)
We have isolated a Pinus sylvestris cDNA encoding a globular protein of 474 amino acids with a predicted molecular weight of 52,995 Da. The deduced amino acid sequence showed 41.9% identity and 13.6% similarity to mammalian cytosolic 3-hydroxy-3-methylglutaryl-CoA-synthase (HMGS). Treatment of Scots pine seedlings with ozone resulted in a transient increase of a 1.95 kb transcript, whereas a 1.2 kb mRNA decreased transiently, indicating a possible influence of ozone on isoprenoid biosynthesis.
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Publication type Article: Journal article
Document type Scientific Article
ISSN (print) / ISBN 0006-3002
Journal Biochimica et Biophysica Acta
Quellenangaben Volume: 1350, Issue: 3, Pages: 247-252
Institute(s) Institute of Biochemical Plant Pathology (BIOP)