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Ratschiller, T.* ; Deutsch, M.A.* ; Calzada-Wack, J. ; Neff, F. ; Roesch, C.* ; Guenzinger, R.* ; Lange, R.* ; Krane, M.*

Heterotopic cervical heart transplantation in mice.

J. Vis. Exp. 102:e52907 (2015)
Open Access Green as soon as Postprint is submitted to ZB.
The heterotopic cervical heart transplantation in mice is a valuable tool in transplant and cardiovascular research. The cuff technique greatly simplifies this model by avoiding challenging suture anastomoses of small vessels thereby reducing warm ischemia time. In comparison to abdominal graft implantation the cervical model is less invasive and the implanted graft is easily accessible for further follow-up examinations. Anastomoses are performed by pulling the ascending aorta of the graft over the cuff with the recipient's common carotid artery and by pulling the main pulmonary artery over the cuff with the external jugular vein. Selection of appropriate cuff size and complete mobilization of the vessels are important for successful revascularization. Ischemia-reperfusion (I/R) injury can be minimized by perfusing the graft with a cardioplegic solution and by hypothermia. In this article, we provide technical details for a simplified and improved cuff technique, which should allow surgeons with basic microsurgical skills to perform the procedure with a high success rate.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Cuff Technique ; Heart ; Heterotopic Heart Transplantation ; Ischemia-reperfusion Injury ; Medicine ; Mice ; Microsurgery ; Transplantation
ISSN (print) / ISBN 1940-087X
e-ISSN 1940-087X
Quellenangaben Volume: 102, Issue: , Pages: , Article Number: e52907 Supplement: ,
Publisher JoVE
Reviewing status Peer reviewed