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Selective plane illumination optical and optoacoustic microscopy for postembryonic imaging.

Laser Photon. Rev. 9, L29-L34 (2015)
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Intravital imaging of large specimens is intrinsically challenging for postembryonic studies. Selective plane illumination microscopy (SPIM) has been introduced to volumetrically visualize organisms used in developmental biology and experimental genetics. Ideally suited for imaging transparent samples, SPIM can offer high frame rate imaging with optical microscopy resolutions and low phototoxicity. However, its performance quickly deteriorates when applied to opaque tissues. To overcome this limitation, SPIM optics were merged with optical and optoacoustic (photoacoustic) readouts. The performance of this hybrid imaging system was characterized using various phantoms and by imaging a highly scattering ex vivo juvenile zebrafish. The results revealed the system's enhanced capability over that of conventional SPIM for high-resolution imaging over extended depths of scattering content. The approach described here may enable future visualization of organisms throughout their entire development, encompassing regimes in which the tissue may become opaque.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Biological Imaging ; Hybrid Imaging Systems ; Light-sheet Microscopy ; Optoacoustic Imaging
ISSN (print) / ISBN 1863-8880
e-ISSN 1863-8899
Quellenangaben Band: 9, Heft: 5, Seiten: L29-L34 Artikelnummer: , Supplement: ,
Verlag Wiley
Verlagsort Weinheim
Begutachtungsstatus Peer reviewed