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Optical mesoscopy without the scatter: Broadband multispectral optoacoustic mesoscopy.

Biomed. Opt. Express 6, 3134-3148 (2015)
Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.
Optical mesoscopy extends the capabilities of biological visualization beyond the limited penetration depth achieved by microscopy. However, imaging of opaque organisms or tissues larger than a few hundred micrometers requires invasive tissue sectioning or chemical treatment of the specimen for clearing photon scattering, an invasive process that is regardless limited with depth. We developed previously unreported broadband optoacoustic mesoscopy as a tomographic modality to enable imaging of optical contrast through several millimeters of tissue, without the need for chemical treatment of tissues. We show that the unique combination of three-dimensional projections over a broad 500 kHz-40 MHz frequency range combined with multi-wavelength illumination is necessary to render broadband multispectral optoacoustic mesoscopy (2B-MSOM) superior to previous optical or optoacoustic mesoscopy implementations.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Multispectral And Hyperspectral Imaging; Photoacoustic Imaging; Photoacoustics; Tomographic Imaging; Spectral Discrimination; High-Resolution; In-Vivo; 3-Dimensional Visualization; Photoacoustic Tomography; Mouse-Brain; Microscopy; Scanner; Ultramicroscopy; Quantification; Reconstruction
ISSN (print) / ISBN 2156-7085
e-ISSN 2156-7085
Quellenangaben Volume: 6, Issue: 9, Pages: 3134-3148 Article Number: , Supplement: ,
Publisher Optical Society of America (OSA)
Reviewing status Peer reviewed