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A synteny-based draft genome sequence of the forage grass Lolium perenne.
Plant J. 84, 816-826 (2015)
Here we report the draft genome sequence of perennial ryegrass (Lolium perenne), an economically important forage and turf grass species widely cultivated in temperate regions worldwide. It is classified along with wheat, barley, oats and Brachypodium distachyon in the Pooideae sub-family of the grass family (Poaceae). Transcriptome data was used to identify 28,455 gene models, and we utilize macro-co-linearity between perennial ryegrass and barley, and synteny within the grass family to establish a synteny-based linear gene order. The gametophytic self-incompatibility (SI) mechanism enables the pistil of a plant to reject self-pollen and therefore promote outcrossing. We have used the sequence assembly to characterise transcriptional changes in the stigma during pollination with both compatible and incompatible pollen. Characterisation of the pollen transcriptome identified homologs to pollen allergens from a range of species, many of which were expressed to very high levels in mature pollen grains, and potentially involved in the SI mechanism. The genome sequence provides a valuable resource for future breeding efforts based on genomic prediction, and will accelerate the development of varieties for more productive grasslands.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Genome Sequence ; Lolium Perenne ; Perennial Ryegrass ; Pollen Allergens ; Self-incompatability