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Genome editing in mice using TALE nucleases.

Methods Mol. Biol. 1338, 229-243 (2016)
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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as sequence-specific nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step without the need for embryonic stem cells. By embryo microinjection of TALEN mRNAs and targeting vectors, knockout and knock-in alleles can be generated fast and efficiently. In this chapter we provide protocols for the application of TALENs in mouse zygotes.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Gene Targeting ; Knock-in ; Knockout ; Mouse ; One-cell Embryo ; Talen
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
ISBN 978-1-4939-2931-3
Konferenztitel TALENs
Quellenangaben Band: 1338, Heft: , Seiten: 229-243 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort Berlin [u.a.]
Begutachtungsstatus Peer reviewed