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Pitchfork and Gprasp2 target smoothened to the primary cilium for Hedgehog pathway activation.

PLoS ONE 11:e0149477 (2016)
Publishers Version DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
as soon as is submitted to ZB.
The seven-transmembrane receptor Smoothened (Smo) activates all Hedgehog (Hh) signaling by translocation into the primary cilia (PC), but how this is regulated is not well understood. Here we show that Pitchfork (Pifo) and the G protein-coupled receptor associated sorting protein 2 (Gprasp2) are essential components of an Hh induced ciliary targeting complex able to regulate Smo translocation to the PC. Depletion of Pifo or Gprasp2 leads to failure of Smo translocation to the PC and lack of Hh target gene activation. Together, our results identify a novel protein complex that is regulated by Hh signaling and required for Smo ciliary trafficking and Hh pathway activation.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Protein-coupled Receptors; Intraflagellar Transport Proteins; Embryonic Stem-cells; Neuronal Cilia; Localization; Mice; Identification; Ciliopathies; Ciliogenesis; Trafficking
Reviewing status