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Fujikawa, Y.* ; Roma, L.P.* ; Sobotta, M.C.* ; Rose, A.J.* ; Berriel Diaz, M. ; Locatelli, G.* ; Breckwoldt, M.O.* ; Misgeld, T.* ; Kerschensteiner, M.* ; Herzig, S. ; Müller-Decker, K.* ; Dick, T.P.*

Mouse redox histology using genetically encoded probes.

Sci. Signal. 9:rs1 (2016)
Verlagsversion Postprint DOI
Open Access Green
Mapping the in vivo distribution of endogenous oxidants in animal tissues is of substantial biomedical interest. Numerous health-related factors, including diet, physical activity, infection, aging, toxins, or pharmacological intervention, may cause redox changes. Tools are needed to pinpoint redox state changes to particular organs, tissues, cell types, and subcellular organelles. We describe a procedure that preserves the in vivo redox state of genetically encoded redox biosensors within histological tissue sections, thus providing "redox maps" for any tissue and comparison of interest. We demonstrate the utility of the technique by visualizing endogenous redox differences and changes in the context of tumor growth, inflammation, embryonic development, and nutrient starvation.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Hydrogen-peroxide; In-vivo; Oxidative Stress; Stem-cells; H2o2; Mitohormesis; Homeostasis; Drosophila; Hyper
ISSN (print) / ISBN 1945-0877
e-ISSN 1937-9145
Zeitschrift Science Signaling
Quellenangaben Band: 9, Heft: 419, Seiten: , Artikelnummer: rs1 Supplement: ,
Verlag American Association for the Advancement of Science (AAAS)
Verlagsort Washington
Begutachtungsstatus Peer reviewed