PuSH - Publikationsserver des Helmholtz Zentrums München

Mülhopt, S.* ; Dilger, M.* ; Diabaté, S.* ; Schläger, C.* ; Krebs, T.* ; Zimmermann, R. ; Buters, J.T.M. ; Oeder, S. ; Wäscher, T.* ; Weiss, C.* ; Paur, H.R.*

Toxicity testing of combustion aerosols at the air-liquid interface with a self-contained and easy-to-use exposure system.

J. Aerosol Sci. 96, 38-55 (2016)
Postprint DOI
Open Access Green
In vitro toxicity testing of airborne particles usually takes place in multi-well plates, where the cells are exposed to a suspension of particles in cell culture medium. Due to the artefacts caused by particle collection and preparation of suspensions, the air-liquid interface (ALI) exposure is challenging this conventional exposure technique to become the method of choice. The ALI technique allows for direct sampling of an aerosol and exposure of cell cultures to airborne particles. At the same time, it reflects the physiological conditions in the lung to a greater extent. So far, the available ALI systems have mostly been laboratory set-ups of the single components. Here, we present a mobile and complete system providing all process technology required for cell exposure experiments at dynamic aerosol sources. The system is controlled by a human machine interface (HMI) with standard routines for experiments and internal testing to assure reproducibility. It also provides documentation of the exposure experiment regarding process parameters and measured doses. The performance of this system is evaluated using fluorescein-sodium dosimetry, which is also used to determine the factor of dose enhancement by optional electrostatic deposition. The application of the system is shown for two different technical aerosol sources: wood smoke particles emitted by a household log wood stove and emissions from a ship diesel engine. After exposure of lung cells, cytotoxicity and gene regulation on a genome-wide scale were analysed.
Weitere Metriken?
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Air-liquid Interface Exposure ; In-vitro ; Lung Cell Culture ; Nanoparticle ; Ship Diesel Emission ; Wood Combustion; In-vitro Exposure; Human Lung-cells; Ambient Particulate Matter; Whole Cigarette-smoke; Epithelial-cells; Diesel Exhaust; Chemical-composition; Nanoparticle Toxicity; Silica Nanoparticles; Particle Aerosols
ISSN (print) / ISBN 0021-8502
e-ISSN 1879-1964
Quellenangaben Band: 96, Heft: , Seiten: 38-55 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort Oxford
Begutachtungsstatus Peer reviewed