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Sahl, S.J.* ; Balzarotti, F.* ; Keller-Findeisen, J.* ; Leutenegger, M.* ; Westphal, V.* ; Egner, A.* ; Lavoie-Cardinal, F.* ; Chmyrov, A. ; Grotjohann, T.* ; Jakobs, S.*

Comment on "Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics".

Science 352:527 (2016)
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Li et al (Research Articles, 28 August 2015, aab3500) purport to present solutions to long-standing challenges in live-cell microscopy, reporting relatively fast acquisition times in conjunction with improved image resolution. We question the methods' reliability to visualize specimen features at sub-100-nanometer scales, because the mandatory mathematical processing of the recorded data leads to artifacts that are either difficult or impossible to disentangle from real features. We are also concerned about the chosen approach of subjectively comparing images from different super-resolution methods, as opposed to using quantitative measures.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Sonstiges: Nachrichtenmeldung
Schlagwörter Fluorescence Microscopy; Optical Resolution; Nanoscopy; Protein; Light; Reveals; Limit; Gfp
ISSN (print) / ISBN 0036-8075
e-ISSN 1095-9203
Zeitschrift Science
Quellenangaben Band: 352, Heft: 6285, Seiten: , Artikelnummer: 527 Supplement: ,
Verlag American Association for the Advancement of Science (AAAS)
Verlagsort Washington
Begutachtungsstatus Peer reviewed