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Ma, W.* ; Gabriel, T.S.* ; Martis, M.M. ; Gursinsky, T.* ; Schubert, V.* ; Vrana, J.* ; Dolezel, J.* ; Grundlach, H. ; Altschmied, L.* ; Scholz, U.* ; Himmelbach, A.* ; Behrens, S.E.* ; Banaei-Moghaddam, A.M.* ; Houben, A.*

Rye B chromosomes encode a functional Argonaute-like protein with in vitro slicer activities similar to its a chromosome paralog.

New Phytol. 213, 916-928 (2016)
Verlagsversion Forschungsdaten DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
B chromosomes (Bs) are supernumerary, dispensable parts of the nuclear genome, which appear in many different species of eukaryote. So far, Bs have been considered to be genetically inert elements without any functional genes. Our comparative transcriptome analysis and the detection of active RNA polymerase II (RNAPII) in the proximity of B chromatin demonstrate that the Bs of rye (Secale cereale) contribute to the transcriptome. In total, 1954 and 1218 B-derived transcripts with an open reading frame were expressed in generative and vegetative tissues, respectively. In addition to B-derived transposable element transcripts, a high percentage of short transcripts without detectable similarity to known proteins and gene fragments from A chromosomes (As) were found, suggesting an ongoing gene erosion process. In vitro analysis of the A- and B-encoded AGO4B protein variants demonstrated that both possess RNA slicer activity. These data demonstrate unambiguously the presence of a functional AGO4B gene on Bs and that these Bs carry both functional protein coding genes and pseudogene copies. Thus, B-encoded genes may provide an additional level of gene control and complexity in combination with their related A-located genes. Hence, physiological effects, associated with the presence of Bs, may partly be explained by the activity of B-located (pseudo)genes.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Argonaute ; B Chromosomes ; B-located Genes ; Secale Cereale ; Gene Erosion ; Gene Expression ; Pseudogenization; Rna-polymerase-ii; Ribosomal-rna; Accessory Chromosomes; Plant Argonautes; Gene-expression; Evolution; Transcription; Pseudogenes; Sequences; Identification
ISSN (print) / ISBN 0028-646X
e-ISSN 1469-8137
Zeitschrift New Phytologist
Quellenangaben Band: 213, Heft: 2, Seiten: 916-928 Artikelnummer: , Supplement: ,
Verlag Wiley
Verlagsort Hoboken
Begutachtungsstatus Peer reviewed