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Studying axonal regeneration by laser microsurgery and high-resolution videomicroscopy.

Methods Mol. Biol. 1451, 271-280 (2016)
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Heterogeneous and unpredictable environmental insult, disease, or trauma can affect the integrity and function of neuronal circuits, leading to irreversible neural dysfunction. The peripheral nervous system can robustly regenerate axons after damage to recover the capacity to transmit sensory information to the brain. The mechanisms that allow axonal repair remain incompletely understood. Here we present a preparation in zebrafish that combines laser microsurgery of sensory axons and videomicroscopy of neurons in multicolor transgenic specimens. This simple protocol allows controlled damage of axons and dynamic high-resolution visualization and quantification of repair.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Fluorescent Protein ; Live Imaging ; Microscopy ; Zebrafish
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
Quellenangaben Band: 1451, Heft: , Seiten: 271-280 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort Berlin [u.a.]
Begutachtungsstatus Peer reviewed