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Diffusion pseudotime robustly reconstructs lineage branching.

Nat. Methods 13, 845-848 (2016)
Postprint DOI
Open Access Green
as soon as is submitted to ZB.
The temporal order of differentiating cells is intrinsically encoded in their single-cell expression profiles. We describe an efficient way to robustly estimate this order according to diffusion pseudotime (DPT), which measures transitions between cells using diffusion-like random walks. Our DPT software implementations make it possible to reconstruct the developmental progression of cells and identify transient or metastable states, branching decisions and differentiation endpoints.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Rna-sequencing Data; Single-cell Data; Expression; Heterogeneity; Trajectories; Progression; Commitment; Embryo; Maps; Stem
Reviewing status