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Gamrekelashvili, J.* ; Giagnorio, R.* ; Jussofie, J.* ; Soehnlein, O.* ; Duchene, J.* ; Briseño, C.G.* ; Ramasamy, S.K.* ; Krishnasamy, K.* ; Limbourg, A.* ; Kapanadze, T.* ; Ishifune, C.* ; Hinkel, R.* ; Radtke, F.* ; Strobl, L.J. ; Zimber-Strobl, U. ; Napp, L.C.* ; Bauersachs, J.* ; Haller, H.* ; Yasutomo, K.* ; Kupatt, C.* ; Murphy, K.M.* ; Adams, R.H.* ; Weber, C.* ; Limbourg, F.P.*

Regulation of monocyte cell fate by blood vessels mediated by Notch signalling.

Nat. Commun. 7:12597 (2016)
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Open Access Gold
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A population of monocytes, known as Ly6C(lo) monocytes, patrol blood vessels by crawling along the vascular endothelium. Here we show that endothelial cells control their origin through Notch signalling. Using combinations of conditional genetic deletion strategies and cell-fate tracking experiments we show that Notch2 regulates conversion of Ly6C(hi) monocytes into Ly6C(lo) monocytes in vivo and in vitro, thereby regulating monocyte cell fate under steady-state conditions. This process is controlled by Notch ligand delta-like 1 (Dll1) expressed by a population of endothelial cells that constitute distinct vascular niches in the bone marrow and spleen in vivo, while culture on recombinant DLL1 induces monocyte conversion in vitro. Thus, blood vessels regulate monocyte conversion, a form of committed myeloid cell fate regulation.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Hematopoietic Stem-cells; Green Fluorescent Protein; Dendritic Cells; Postnatal Arteriogenesis; Classical Monocytes; Lineage Commitment; Angiocrine Factors; Endothelial-cells; Vascular Niche; Lymph-nodes
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Quellenangaben Volume: 7, Issue: , Pages: , Article Number: 12597 Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Reviewing status Peer reviewed