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CRISPR-Cas9 enables conditional mutagenesis of challenging loci.

Sci. Rep. 6:32326 (2016)
Publishers Version Research data DOI PMC
Open Access Gold
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The International Knockout Mouse Consortium (IKMC) has produced a genome-wide collection of 15,000 isogenic targeting vectors for conditional mutagenesis in C57BL/6N mice. Although most of the vectors have been used successfully in murine embryonic stem (ES) cells, there remain a set of nearly two thousand genes that have failed to target even after several attempts. Recent attention has turned to the use of new genome editing technology for the generation of mutant alleles in mice. Here, we demonstrate how Cas9-assisted targeting can be combined with the IKMC targeting vector resource to generate conditional alleles in genes that have previously eluded targeting using conventional methods.
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Publication type Article: Journal article
Document type Scientific Article
Keywords One-step Generation; Homologous Recombination; Gene-function; Genome; Cas9; Consortium; Resource; System
Reviewing status