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Dual function of NRP1 in axon guidance and subcellular target recognition in cerebellum.
Neuron 91, 1276-1291 (2016)
Publ. Version/Full Text Research data DOI
Subcellular target recognition in the CNS is the culmination of a multiple-step program including axon guidance, target recognition, and synaptogenesis. In cerebellum, basket cells (BCs) innervate the soma and axon initial segment (AIS) of Purkinje cells (PCs) to form the pinceau synapse, but the underlying mechanisms remain incompletely understood. Here, we demonstrate that neuropilin-1 (NRP1), a Semaphorin receptor expressed in BCs, controls both axonal guidance and subcellular target recognition. We show that loss of Semaphorin 3A function or specific deletion of NRP1 in BCs alters the stereotyped organization of BC axon and impairs pinceau synapse formation. Further, we identified NRP1 as a trans-synaptic binding partner of the cell adhesion molecule neurofascin-186 (NF186) expressed in the PC AIS during pinceau synapse formation. These findings identify a dual function of NRP1 in both axon guidance and subcellular target recognition in the construction of GABAergic circuitry.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Cell-adhesion Molecule; Initial Segment; Synaptic Connections; Semaphorin 3a; Neurofascin; L1; Interneurons; Gene; Neuropilin-1; Inhibition
ISSN (print) / ISBN 0896-6273
Quellenangaben Volume: 91, Issue: 6, Pages: 1276-1291
Publisher Cell Press
Publishing Place Cambridge, Mass.
Reviewing status Peer reviewed
Institute(s) Institute of Developmental Genetics (IDG)