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Madreiter-Sokolowski, C.T.* ; Klec, C.* ; Parichatikanond, W.* ; Stryeck, S.* ; Gottschalk, B.* ; Pulido, S.* ; Rost, R.* ; Eroglu, E.* ; Hofmann, N.A.* ; Bondarenko, A.I.* ; Madl, T. ; Waldeck-Weiermair, M.* ; Malli, R.* ; Graier, W.F.*

PRMT1-mediated methylation of MICU1 determines the UCP2/3 dependency of mitochondrial Ca2+ uptake in immortalized cells.

Nat. Commun. 7:12897 (2016)
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Open Access Gold
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Recent studies revealed that mitochondrial Ca2+ channels, which control energy flow, cell signalling and death, are macromolecular complexes that basically consist of the pore-forming mitochondrial Ca2+ uniporter (MCU) protein, the essential MCU regulator (EMRE), and the mitochondrial Ca2+ uptake 1 (MICU1). MICU1 is a regulatory subunit that shields mitochondria from Ca2+ overload. Before the identification of these core elements, the novel uncoupling proteins 2 and 3 (UCP2/3) have been shown to be fundamental for mitochondrial Ca2+ uptake. Here we clarify the molecular mechanism that determines the UCP2/3 dependency of mitochondrial Ca2+ uptake. Our data demonstrate that mitochondrial Ca2+ uptake is controlled by protein arginine methyl transferase 1 (PRMT1) that asymmetrically methylates MICU1, resulting in decreased Ca2+ sensitivity. UCP2/3 normalize Ca2+ sensitivity of methylated MICU1 and, thus, re-establish mitochondrial Ca2+ uptake activity. These data provide novel insights in the complex regulation of the mitochondrial Ca2+ uniporter by PRMT1 and UCP2/3.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Protein Arginine Methyltransferases; Calcium Uniporter; Essential Component; Oxidative Stress; Breast-cancer; Mcu; Complex; Expression; Membrane; Binding
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Quellenangaben Volume: 7, Issue: , Pages: , Article Number: 12897 Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Reviewing status Peer reviewed