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Exhaust air particle PCR detects helicobacter hepaticus infections at low prevalence.

J. Veterinar. Sci. Technolo. 7:1000343 (2016)
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Reliable detection of unwanted organisms in experimental animal colonies is an essential requirement for biomedical research. Since most rodents are housed in Individually Ventilated Cage (IVC) systems which prevent aerogenic transmission of pathogens, soiled-bedding sentinels are commonly used for routine health monitoring. In this study, we investigated whether testing of Exhaust Air Particle (EAP) samples by real-time PCR (EAP PCR) might be a valid alternative to SBS for the detection of H. hepaticus infections. EAP PCR detected H. hepaticus infections at a minimal prevalence of 1/63 cages within one week. EAP PCR provides a sensitive, simple, and reliable alternative to SBS for H. hepaticus identification in laboratory mouse colonies.
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Publication type Article: Journal article
Document type Scientific Article
e-ISSN 2157-7579
Quellenangaben Volume: 7, Issue: 4, Pages: , Article Number: 1000343 Supplement: ,
Publisher OMICS
Publishing Place Los Angeles, Calif.
Reviewing status Peer reviewed