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Hofmann, K.* ; Fiedler, S.* ; Vierkotten, S. ; Weber, J.* ; Klee, S. ; Jia, J. ; Zwickenpflug, W.* ; Flockerzi, V.* ; Storch, U.* ; Yildirim, A.Ö. ; Gudermann, T.* ; Königshoff, M. ; Dietrich, A.*

Classical transient receptor potential 6 (TRPC6) channels support myofibroblast differentiation and development of experimental pulmonary fibrosis.

Biochim. Biophys. Acta 1863, 560-568 (2017)
Open Access Green as soon as Postprint is submitted to ZB.
Pulmonary fibrosis (PF) is a chronic progressive lung disease without effective medical treatment options leading to respiratory failure and death within 3–5 years of diagnosis. The pathological process of PF is driven by aberrant wound-healing involving fibroblasts and myofibroblasts differentiated by secreted profibrotic transforming growth factor β (TGF-β1). Classical transient receptor potential 6 (TRPC6), a Na+- and Ca2 +-permeable cation channel, is able to promote myofibroblast conversion of primary rat cardiac and human dermal fibroblasts and TRPC6-deficiency impaired wound healing after injury. To study a potential role of TRPC6 in the development of PF we analyzed lung function, gene and protein expression in wild-type (WT) and TRPC6-deficient (TRPC6 −/−) lungs utilizing a bleomycin-induced PF-model. Fibrotic WT-mice showed a significant higher death rate while bleomycin-treated TRPC6-deficient mice were partly protected from fibrosis as a consequence of a lower production of collagen and an almost normal function of the respiratory system (reduced resistance and elastance compared to fibrotic WT-mice). On a molecular level TGF-β1 induced TRPC6 up-regulation, increased Ca2 + influx and nuclear NFAT localization in WT primary murine lung fibroblasts (PMLFs) resulting in higher stress fiber formation and accelerated contraction rates as compared to treated TRPC6-deficient fibroblasts. Therefore, we conclude that TRPC6 is an important determinant for TGF-β1-induced myofibroblast differentiation during fibrosis and specific channel inhibitors might be beneficial in a future treatment of PF.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Cell Contraction ; Myofibroblast Differentiation ; Primary Murine Lung Fibroblasts ; Pulmonary Fibrosis ; Tgf-β1 ; Trpc6; Cation Channel; Mesenchymal Transition; Extracellular-matrix; Lung Fibrosis; G-protein; In-vivo; Activation; Cells; Disease; Mice
ISSN (print) / ISBN 0006-3002
Quellenangaben Volume: 1863, Issue: 2, Pages: 560-568 Article Number: , Supplement: ,
Publisher Elsevier
Publishing Place Amsterdam