Open Access Green as soon as Postprint is submitted to ZB.
Epigenetics and control of RNAs.
Methods Mol. Biol. 1488, 217-237 (2017)
Histone modifications are epigenetic marks that fundamentally impact the regulation of gene expression. Integrating histone modification information in the analysis of gene expression traits (eQTL mapping) has been shown to significantly enhance the prediction of eQTLs. In this chapter, we describe (1) how to perform quantitative trait locus (QTL) analysis using histone modification levels as traits and (2) how to integrate these data with information on RNA expression for the elucidation of the epigenetic control of transcript levels. We will provide a comprehensive introduction into the topic, describe in detail how ChIP-seq data are analyzed and elaborate on how to integrate ChIP-seq and RNA-seq data from a segregating disease animal model for the identification of the epigenetic control of RNA expression.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Chip-seq ; Histone Modifications ; Integrative Analysis ; Qtl Mapping ; Recombinant Inbred Panel ; Rna Expression
ISSN (print) / ISBN 1064-3745
Book Volume Title Systems Genetics
Journal Methods in Molecular Biology
Quellenangaben Volume: 1488, Pages: 217-237
Publishing Place Berlin [u.a.]
Institute(s) Institute of Computational Biology (ICB)