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Bozhko, D. ; Osborn, E.A.* ; Rosenthal, A. ; Verjans, J.W.* ; Hara, T.* ; Kellnberger, S. ; Wissmeyer, G. ; Ovsepian, S.V. ; McCarthy, J.R.* ; Mauskapf, A. ; Stein, A.F. ; Jaffer, F.A.* ; Ntziachristos, V.

Quantitative intravascular biological fluorescence-ultrasound imaging of coronary and peripheral arteries in vivo.

Eur. Heart J. Cardiovasc. Imaging 18, 1253-1261 (2017)
Verlagsversion Forschungsdaten DOI
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Aims: (i) to evaluate a novel hybrid near-infrared fluorescence - intravascular ultrasound (NIRF-IVUS) system in coronary and peripheral swine arteries in vivo; (ii) to assess simultaneous quantitative biological and morphological aspects of arterial disease. Methods and results: Two 9F/15MHz peripheral and 4.5F/40MHz coronary near-infrared fluorescence (NIRF)-IVUS catheters were engineered to enable accurate co-registrtation of biological and morphological readings simultaneously in vivo. A correction algorithm utilizing IVUS information was developed to account for the distance-related fluorescence attenuation due to through-blood imaging. Corrected NIRF (cNIRF)-IVUS was applied for in vivo imaging of angioplasty-induced vascular injury in swine peripheral arteries and experimental fibrin deposition on coronary artery stents, and of atheroma in a rabbit aorta, revealing feasibility to intravascularly assay plaque structure and inflammation. The addition of ICG-enhanced NIRF assessment improved the detection of angioplasty-induced endothelial damage compared to standalone IVUS. In addition, NIRF detection of coronary stent fibrin by in vivo cNIRF-IVUS imaging illuminated stent pathobiology that was concealed on standalone IVUS. Fluorescence reflectance imaging and microscopy of resected tissues corroborated the in vivo findings. Conclusions: Integrated cNIRF-IVUS enables simultaneous co-registered through-blood imaging of disease related morphological and biological alterations in coronary and peripheral arteries in vivo. Clinical translation of cNIRF-IVUS may significantly enhance knowledge of arterial pathobiology, leading to improvements in clinical diagnosis and prognosis, and helps to guide the development of new therapeutic approaches for arterial diseases.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Fluorescence Imaging ; Ivus ; Molecular Imaging ; Nirf; Near-infrared Fluorescence; Optical Coherence Tomography; Cardiovascular-disease; Atherosclerosis; Challenges; Catheter; Therapy; Humans; Risk; Ivus
ISSN (print) / ISBN 2047-2404
e-ISSN 2047-2412
Quellenangaben Band: 18, Heft: 11, Seiten: 1253-1261 Artikelnummer: , Supplement: ,
Verlag Oxford University Press
Verlagsort Oxford
Begutachtungsstatus Peer reviewed