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Morgenstern, J.* ; Fleming, T.* ; Schumacher, D.* ; Eckstein, V.* ; Freichel, M.* ; Herzig, S. ; Nawroth, P.P.

Loss of glyoxalase 1 induces compensatory mechanism to achieve dicarbonyl detoxification in mammalian Schwann cells.

J. Biol. Chem. 292, 3224-3238 (2016)
Publ. Version/Full Text Postprint Research data DOI
Open Access Green
The glyoxalase system is a highly specific enzyme system existing in all mammalian cells which is responsible for the detoxification of dicarbonyl species, primarily methylglyoxal (MG). It has been implicated to play an essential role in preventing the increased formation of advanced glycation endproducts under certain pathological conditions. We have established the first glyoxalase 1 knock-out model (GLO1-/-) in mammalian Schwann cells using CRISPR/Cas9 technique to investigate compensatory mechanisms. Neither elevated concentrations of MG nor associated protein modifications were observed in GLO1-/- cells. Alternative detoxification of MG in GLO1-/- is achieved by increased catalytic efficiency of aldose reductase towards hemithioacetal (product of glutathione and MG), most likely caused by S-nitrosylation of aldose reductase. The hemithioacetal is mainly converted into lactaldehyde, which is paralleled by a loss of reduced glutathione. Inhibition of aldose reductase in GLO1-/- cells is associated with an increased sensitivity against MG, elevated intracellular MG levels and associated modifications, as well as increased oxidative stress. Our data suggest that aldose reductase can compensate the loss of GLO1. This might be of clinical importance within the context of neuronal diseases caused by an impaired glyoxalase system and elevated levels of dicarbonyl species, such as MG.
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Publication type Article: Journal article
Document type Scientific Article
Keywords cell metabolism; CRISPR/Cas; diabetes; enzyme kinetics; glycation; toxicity; aldose reductase; diabetic neuropathy; glyoxalase system; methylglyoxal; Glycation End-products; Aldose Reductase Inhibitor; Diabetic-neuropathy; Nitric-oxide; Endothelial-cells; Mass-spectrometry; Methylglyoxal; System; Complications; Proteins
ISSN (print) / ISBN 0021-9258
e-ISSN 1083-351X
Quellenangaben Volume: 292, Issue: 8, Pages: 3224-3238 Article Number: , Supplement: ,
Publisher American Society for Biochemistry and Molecular Biology
Publishing Place Bethesda
Reviewing status Peer reviewed