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Nasr, M.L.* ; Baptista, D.* ; Strauss, M.* ; Sun, Z.J.* ; Grigoriu, S.* ; Huser, S.* ; Plueckthun, A.* ; Hagn, F. ; Walz, T.* ; Hogle, J.M.* ; Wagner, G.*

Covalently circularized nanodiscs for studying membrane proteins and viral entry.

Nat. Methods 14, 49-52 (2017)
Verlagsversion Postprint DOI
Open Access Green
We engineered covalently circularized nanodiscs (cNDs) which, compared with standard nanodiscs, exhibit enhanced stability, defined diameter sizes and tunable shapes. Reconstitution into cNDs enhanced the quality of nuclear magnetic resonance spectra for both VDAC-1, a beta-barrel membrane protein, and the G-protein-coupled receptor NTR1, an alpha-helical membrane protein. In addition, we used cNDs to visualize how simple, nonenveloped viruses translocate their genomes across membranes to initiate infection.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Phospholipid-bilayer Nanodiscs; Scaffold Proteins; Cell Entry; Poliovirus; Receptor; Reconstitution; Particles; Virus
ISSN (print) / ISBN 1548-7091
e-ISSN 1548-7105
Zeitschrift Nature Methods
Quellenangaben Band: 14, Heft: 1, Seiten: 49-52 Artikelnummer: , Supplement: ,
Verlag Nature Publishing Group
Verlagsort New York, NY
Begutachtungsstatus Peer reviewed