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Time-specific effects of spindle positioning on embryonic progenitor pool composition and adult neural stem cell seeding.

Neuron 93, 777-791.e3 (2017)
Verlagsversion Forschungsdaten DOI
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
The developmental mechanisms regulating the number of adult neural stem cells (aNSCs) are largely unknown. Here we show that the cleavage plane orientation in murine embryonic radial glia cells (RGCs) regulates the number of aNSCs in the lateral ganglionic eminence (LGE). Randomizing spindle orientation in RGCs by overexpression of Insc or a dominant-negative form of Lgn (dnLgn) reduces the frequency of self-renewing asymmetric divisions while favoring symmetric divisions generating two SNPs. Importantly, these changes during embryonic development result in reduced seeding of aNSCs. Interestingly, no effects on aNSC numbers were observed when Insc was overexpressed in postnatal RGCs or aNSCs. These data suggest a new mechanism for controlling aNSC numbers and show that the role of spindle orientation during brain development is highly time and region dependent.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Insc ; Lgn ; Sez ; Adult Neural Stem Cells ; Division Plane ; Radial Glial Cells ; Short Neural Precursor; Subventricular Zone; In-vivo; Olfactory-bulb; Vascular Niche; Self-renewal; Neuroepithelial Cells; Planar Divisions; Radial Glia; Par-complex; Neurogenesis
ISSN (print) / ISBN 0896-6273
e-ISSN 1097-4199
Zeitschrift Neuron
Quellenangaben Band: 93, Heft: 4, Seiten: 777-791.e3 Artikelnummer: , Supplement: ,
Verlag Cell Press
Verlagsort Cambridge, Mass.
Begutachtungsstatus Peer reviewed