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Steens, J.* ; Zuk, M.* ; Benchellal, M.* ; Bornemann, L.* ; Teichweyde, N.* ; Hess-Rieger, J. ; Unger, K. ; Görgens, A.* ; Klump, H.* ; Klein, D.*

In vitro generation of vascular wall-resident multipotent stem cells of mesenchymal nature from murine induced pluripotent stem cells.

Stem Cell Rep. 8, 919-932 (2017)
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Open Access Gold
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The vascular wall (VW) serves as a niche for mesenchymal stem cells (MSCs). In general, tissue-specific stem cells differentiate mainly to the tissue type from which they derive, indicating that there is a certain code or priming within the cells as determined by the tissue of origin. Here we report the in vitro generation of VW-typical MSCs from induced pluripotent stem cells (iPSCs), based on a VW-MSC-specific gene code. Using a lentiviral vector expressing the so-called Yamanaka factors, we reprogrammed tail dermal fibroblasts from transgenic mice containing the GFP gene integrated into the Nestin-locus (NEST-iPSCs) to facilitate lineage tracing after subsequent MSC differentiation. A lentiviral vector expressing a small set of recently identified human VW-MSC-specific HOX genes then induced MSC differentiation. This direct programming approach successfully mediated the generation of VW-typical MSCs with classical MSC characteristics, both in vitro and in vivo.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Hox Gene ; Direct Programming ; Induced Pluripotent Stem Cells ; Nestin ; Vascular Wall-derived Mesenchymal Stem Cells; Human Bone-marrow; Stromal Cells; Different Organs; Adipose-tissue; Cord Blood; Hox Codes; Adult; Fetal; Differentiation; Expression
ISSN (print) / ISBN 2213-6711
Quellenangaben Volume: 8, Issue: 4, Pages: 919-932 Article Number: , Supplement: ,
Publisher Cell Press
Publishing Place Maryland Heights, MO
Reviewing status Peer reviewed