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Spinal poly-GA inclusions in a C9orf72 mouse model trigger motor deficits and inflammation without neuron loss.
Acta Neuropathol. 134, 241–254 (2017)
Translation of the expanded (ggggcc)n repeat in C9orf72 patients with amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) causes abundant poly-GA inclusions. To elucidate their role in pathogenesis, we generated transgenic mice expressing codon-modified (GA)149 conjugated with cyan fluorescent protein (CFP). Transgenic mice progressively developed poly-GA inclusions predominantly in motoneurons and interneurons of the spinal cord and brain stem and in deep cerebellar nuclei. Poly-GA co-aggregated with p62, Rad23b and the newly identified Mlf2, in both mouse and patient samples. Consistent with the expression pattern, 4-month-old transgenic mice showed abnormal gait and progressive balance impairment, but showed normal hippocampus-dependent learning and memory. Apart from microglia activation we detected phosphorylated TDP-43 but no neuronal loss. Thus, poly-GA triggers behavioral deficits through inflammation and protein sequestration that likely contribute to the prodromal symptoms and disease progression of C9orf72 patients.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Als ; C9orf72 ; Ftd ; Ftld ; Mnd ; Mouse Model ; Neurodegeneration ; Neurological Disorder; Dipeptide-repeat Proteins; Frontotemporal Dementia; Hexanucleotide Repeat; Transgenic Mice; Startle Reflex; Rna Foci; Toxicity; Pathology; Als/ftd; Als
ISSN (print) / ISBN 0001-6322
e-ISSN 1432-0533
Zeitschrift Acta Neuropathologica
Quellenangaben Band: 134, Heft: 2, Seiten: 241–254 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort New York
Begutachtungsstatus peer-reviewed