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Peng, T. ; Thorn, K.* ; Schroeder, T.* ; Wang, L.* ; Theis, F.J. ; Marr, C. ; Navab, N.*

A BaSiC tool for background and shading correction of optical microscopy images.

Nat. Commun. 8:14836 (2017)
Publ. Version/Full Text Research data DOI
Open Access Gold
Creative Commons Lizenzvertrag
Quantitative analysis of bioimaging data is often skewed by both shading in space and background variation in time. We introduce BaSiC, an image correction method based on low-rank and sparse decomposition which solves both issues. In comparison to existing shading correction tools, BaSiC achieves high-accuracy with significantly fewer input images, works for diverse imaging conditions and is robust against artefacts. Moreover, it can correct temporal drift in time-lapse microscopy data and thus improve continuous single-cell quantification. BaSiC requires no manual parameter setting and is available as a Fiji/ImageJ plugin.
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Publication type Article: Journal article
Document type Scientific Article
Keywords High-throughput Microscopy; Minimization
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Quellenangaben Volume: 8, Issue: , Pages: , Article Number: 14836 Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Reviewing status Peer reviewed