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Sonntag, M. ; Jagtap, P.K. ; Simon, B.* ; Appavou, M.S.* ; Geerlof, A. ; Stehle, R.* ; Gabel, F.* ; Hennig, J. ; Sattler, M.

Segmental, domain-selective perdeuteration and small-angle neutron scattering for structural analysis of multi-domain proteins.

Angew. Chem.-Int. Edit. 56, 9322-9325 (2017)
Postprint Research data DOI
Open Access Green
Multi-domain proteins play critical roles in fine-tuning essential processes in cellular signaling and gene regulation. Typically, multiple globular domains that are connected by flexible linkers undergo dynamic rearrangements upon binding to protein, DNA or RNA ligands. RNA binding proteins (RBPs) represent an important class of multi-domain proteins, which regulate gene expression by recognizing linear or structured RNA sequence motifs. Here, we employ segmental perdeuteration of the three RNA recognition motif (RRM) domains in the RBP TIA-1 using SortaseA mediated protein ligation. We show that domain-selective perdeuteration combined with contrast-matched small-angle neutron scattering (SANS), SAXS and computational modeling provides valuable information to precisely define relative domain arrangements. The approach is generally applicable to study conformational arrangements of individual domains in multi-domain proteins and changes induced by ligand binding.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Nmr Spectroscopy ; Perdeuteration ; Protein Ligation ; Small-angle Neutron Scattering ; X-ray Diffraction
ISSN (print) / ISBN 1433-7851
e-ISSN 1521-3773
Quellenangaben Volume: 56, Issue: 32, Pages: 9322-9325 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place Weinheim
Reviewing status Peer reviewed