PuSH - Publikationsserver des Helmholtz Zentrums München

Mattii, M. ; Lovászi, M.* ; Garzorz, N.V.* ; Atenhan, A. ; Quaranta, M. ; Lauffer, F.* ; Konstantinow, A.* ; Küpper, M. ; Zouboulis, C.C.* ; Kemeny, L.* ; Eyerich, K.* ; Schmidt-Weber, C.B. ; Törőcsik, D.* ; Eyerich, S.

Sebocytes contribute to skin inflammation by promoting the differentiation of T helper 17 cells.

Br. J. Dermatol. 178, 722-730 (2017)
Postprint Forschungsdaten DOI
Open Access Green
BackgroundThe main function of sebocytes is considered to be the production of lipids to moisturize the skin. However, it recently became apparent that sebocytes release chemokines and cytokines and respond to proinflammatory stimuli as well as the presence of bacteria. ObjectivesTo analyse the functional communication between human sebocytes and T cells. MethodsImmunofluorescence stainings for CD4 and interleukin (IL)-17 were performed on acne sections and healthy skin. Migration assays and T-cell-stimulation cultures were performed with supernatants derived from unstimulated or prestimulated SZ95 sebocytes. Dendritic cells were generated in the presence of SZ95 supernatant and subsequently used in mixed leucocyte reactions. ResultsWe showed that CD4(+) IL-17(+) T cells accumulate around the pilosebaceous unit and are in close contact with sebocytes in acne lesions. By using SZ95 sebocyte supernatant, we demonstrate a chemotactic effect of sebocytes on neutrophils, monocytes and T cells in a CXCL8-dependent manner. Furthermore, sebocyte supernatant induces the differentiation of CD4(+) CD45RA(+) naive T cells into T helper (Th)17 cells via the secretion of IL-6, transforming growth factor- and, most importantly, IL-1. No direct effects of sebocytes on the function of CD4(+) CD45RO(+) memory T cells were detected. Moreover, sebocytes functionally interact with Propionibacterium acnes in the maturation of dendritic cells, leading to antigen-presenting cells that preferentially prime Th17 cells. ConclusionsOur study provides evidence that human sebocytes actively participate in inflammatory processes in the skin by recruiting and communicating with immune cells. This interaction leads to the generation of Th17 cells, which might contribute to the pathogenesis not only of acne vulgaris, but also of several inflammatory skin diseases. What's already known about this topic? Sebocytes are part of the pilosebaceous unit and produce lipids for moisturizing the skin. They were long regarded as bystander cells during skin inflammation with no impact on the immune response. What does this study add? We show that sebocytes actively contribute to inflammatory processes by recruitment of immune cells into the skin and by skewing T-cell differentiation towards T helper 17 cells. What is the translational message? This interaction of sebocytes might be important in the pathogenesis of other inflammatory diseases. Plain language summary available online Respond to this article
Weitere Metriken?
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Propionibacterium-acnes; Antimicrobial Peptides; Dendritic Cells; Atopic-dermatitis; In-vitro; Expression; Vulgaris; Il-17; Responses; Keratinocytes
ISSN (print) / ISBN 0007-0963
e-ISSN 1365-2133
Quellenangaben Band: 178, Heft: 3, Seiten: 722-730 Artikelnummer: , Supplement: ,
Verlag Wiley
Verlagsort Hoboken
Begutachtungsstatus Peer reviewed