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Danner, E.* ; Bashir, S.* ; Yumlu, S.* ; Wurst, W. ; Wefers, B. ; Kuehn, R.*

Control of gene editing by manipulation of DNA repair mechanisms.

Mamm. Genome 28, 262-274 (2017)
Open Access Green as soon as Postprint is submitted to ZB.
DNA double-strand breaks (DSBs) are produced intentionally by RNA-guided nucleases to achieve genome editing through DSB repair. These breaks are repaired by one of two main repair pathways, classic non-homologous end joining (c-NHEJ) and homology-directed repair (HDR), the latter being restricted to the S/G2 phases of the cell cycle and notably less frequent. Precise genome editing applications rely on HDR, with the abundant c-NHEJ formed mutations presenting a barrier to achieving high rates of precise sequence modifications. Here, we give an overview of HDR- and c-NHEJ-mediated DSB repair in gene editing and summarize the current efforts to promote HDR over c-NHEJ.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Double-strand Breaks; Homology-directed Repair; Pluripotent Stem-cells; End Resection; Crispr-cas9 Nucleases; Knock-in; Muscular-dystrophy; Mammalian-cells; Mouse Model; Donor Dna
ISSN (print) / ISBN 0938-8990
e-ISSN 1432-1777
Quellenangaben Volume: 28, Issue: 7-8, Pages: 262-274 Article Number: , Supplement: ,
Publisher Springer
Publishing Place New York
Reviewing status Peer reviewed